Purification of avian influenza virus (H9N2) from allantoic fluidby size-exclusion chromatography

Isolation and Identification of Avian Influenza Virus H9N2 Subtype

Isolation of avian influenza virus (H9N2) from emu in China

This is the first reported isolation of avian influenza virus (AIV) from emu in China. An outbreak of AIV infection occurred at an emu farm that housed 40 four-month-old birds. Various degrees of haemorrhage were discovered in the tissues of affected emus. Cell degeneration and necrosis were observed microscopically. Electron microscopy revealed round or oval virions with a diameter of 80 nm to...

Effects of dietary supplementation of mannan-oligosaccharide on virus shedding in avian influenza (H9N2) challenged broilers

Avian influenza (AI) is a highly contagious disease causing significant economic losses worldwide. The aim of this study is to evaluate the effect of mannan-oligosaccharide (MOS) on tracheal and cloacal virus shedding in AI challenged broilers and contamination of environment with H9N2. A total of 300 1-day-old-broiler chicks were randomly divided into 3 groups (A, B and C) and supplemented 0.2...

Vertical Transmission of H9N2 Avian Influenza Virus in Goose

During a study on high mortality cases of goose embryo in Shandong Province, China (2014-2015), we isolated an H9N2 avian influenza virus (AIV) strain (A/goose/Shandong/DP01/2014, DP01), which was supposedly the causative agent for goose embryo death. Sequence analysis revealed that DP01 shared 99.9% homology in the HA gene with a classic immune suppression strain SD06. To study the potential v...

Immunity Against Avian Influenza Viruspanax Ginseng Polysaccharide (GPS) Can Improve Immunity Against H9N2 Avian Influenza Virus in Chickens

The humoral immunization potential of panax ginseng polysaccharide (GPS) against H9N2 avian influenza virus (H9N2 AIV) in chickens was investigated. The effects of GPS treatment before and during H9N2 AIV infection were determined in chicken embryo fibroblast (CEF) by MTT (3(4, 5-dimethylthiazol-2-yl)-2, 3-diphenyl tetrazolium bromide) assays and quantitative RT-PCR analysis of MHC and cytokine...